Genomics Education Partnership

Next Generation Sequencing Video Tour

A Tour of Next Generation Sequencing: Inside the Washington University Genome Center is a set of interviews with three Directors from the Genome Center. The interviews are interspersed with video shots and still images of the machines in action and animations to explain the chemistry of next generation sequencing. The video is broken up into four parts:


  • Introduction to next-gen sequencing

    Introduction with Elaine Mardis, Co-Director of the Genome Center: progress, an overview, production statistics, and uses of new sequencing technologies.

  • introduction to illumina

    Illumina with Cherilynn Shadding, Outreach Director of the Genome Center: Illumina sequencing technology (including bridge amplification, reversible terminator synthesis, paired end reads, problems) and Illumina production statistics.

  • introduction to 454

    454 with Cherilynn Shadding, Outreach Director of the Genome Center: 454 sequencing technology (including emulsion PCR, pyrosequencing, paired end reads, problems), 454 production statistics, and comparisons of 454 and Illumina technologies.

  • next gen sequencing applications

    Applications with Dr. Rick Wilson, Director of the Genome Center: current projects (including new genomes, microbial genomes, the 1000 Genome Project, cancer genome sequencing) and implications of genome sequencing/GINA (the Genetic Information Nondiscrimination Act).

About the Video Tour

This video is aimed at informing biology students of new advances in genome sequencing technologies and applications. Students may understand this video better after they have watched the previous video (Sequencing a Genome: Inside the Washington University Genome Center). This video can be used with advanced high school students or at the undergraduate or beginning graduate levels.

454 machine

The video was produced by a partnership between Washington University Genome Center and Washington University Department of Biology.

Illumina animation

Additional Materials

Loading Illumina machine

The video clips and supplemental materials are available for download on the Tour Materials page. Additional supplemental materials include the full script, tables with machine comparisons, a glossary, Powerpoint presentations of animations shown in the videos, and a complete list of image sources.

Funding and Support

Loading Illumina machine

This project was funded by a Professorship Award for Dr. Sarah C.R. Elgin from the Howard Hughes Medical Institute (HHMI) to Washington University. Additional support was provided by the National Human Genome Research Institute (NHGRI) through its funding of the Washington University Genome Center Outreach Program.

The tour videos and associated materials are copyright 2010, Washington University in St. Louis. Teachers may copy materials as needed for classroom use.

Copyright 2006-2014, Washington University in St. Louis. Legal Notices

Last Update: 06/29/2014.