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Home / Curriculum / Course Materials - WU / Annotation / Specific Issues in GEP Annotation ProjectsSpecific Issues in GEP Annotation Projects
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The following documents describe specific issues pertinent to GEP annotation projects.
Specific Issues in GEP Annotation Projects
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Annotation for D. virilis
Last update: 12/26/2018
This is a PowerPoint presentation describing the recommended strategies for annotating a D. virilis fosmid. The homology-based annotation strategy should also be applicable to annotation of D. erecta and D. mojavensis projects.
Tags: Annotation Strategy -
Annotating Splice Sites: Workflow
Last update: 08/17/2018
A one-page summary of the logic process for identifying appropriate splice sites when annotating.
Download links: PDF -
GEP Annotation Report
Last update: 12/30/2018
This document is the revised annotation report that GEP students will use to report their annotation results to the GEP.
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Quick Check of Student Annotations
Last update: 12/20/2017
This document describes a protocol that GEP faculty can use to quickly identify potential errors in student gene annotations.
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Annotation Instruction Sheet
Last update: 12/27/2018
This document is a more in depth description of the evidence based annotation technique used by the GEP. This document is designed to complement and extend the basic technique described in the Annotation for D. virilis powerpoint.
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Annotation Strategy Guide
Last update: 12/29/2018
This document illustrates how the strategies outlined in the Annotation Instruction Sheet can be applied to more challenging annotation cases.
Tags: Annotation Strategy -
Drosophila Annotation Goals: Final Presentation and Written Reports
Last update: 08/06/2014
This document describes the primary annotation goals to be included in the final oral presentation and written report for students enrolled in the Bio4342 course at WU.
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About Synteny Analysis
Last update: 12/27/2018
A one-page summary of the logic process used to determine if genes in a fosmid is syntenic with respect to D. melanogaster.
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Web Databases for Drosophila
Last update: 12/26/2018
This PowerPoint presentation introduces the species-specific databases available on the web, including Flybase and the Ensembl Drosophila melanogaster database.
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Primer on Reading Frames and Phase
Last update: 08/16/2016
This primer provides an overview on how to determine the phase of donor and acceptor splice sites.
