About This Page
To prepare to annotate genes, students are first introduced to the common tools available for annotation (BLAST, RepeatMasker, UCSC Genome Browser). This page contains a list of annotation tutorials developed by GEP faculty.
List of Annotation Tutorials and Walkthroughs
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Annotation Resources
Dr. Gary Kuleck (Loyola Marymount University) has posted a set of annotation materials protocols based on his students work on annotation of Drosophila erecta.
- Last update: 01/20/2007
- Tags: BLAST Browser Gene finding
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Generating Multiple Sequence Alignments with ClustalW
Dr. Susan Parrish developed a basic lecture and weblem exercise (found at the end of the lecture) on using ClustalW to generate multiple sequence alignments, phylograms, and cladograms. This lecture and exercise are given prior to beginning the GEP annotation projects. Students who submit their GEP annotation projects early are then asked to generate multiple sequence alignments and phylograms of the putative proteins encoded within their assigned contig or fosmid, compared to those related proteins encoded by other Drosophila species of interest to the GEP.
- Last update: 04/03/2011
- Tags: ClustalW Multiple sequence alignment
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FlyBase for Undergrads
Dr. Hui-Min Chung (University of West Florida) and her students have developed an instructional video aimed at introducing undergraduate students to the research tools available at FlyBase.
Download links:
YouTube Video- Last update: 02/12/2011
- Tags: FlyBase
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Gene Annotation of Genomic DNA
Dr. James Bedard (Adams State College) with students Amber Harlan and Joshua Machone presented a walkthrough on their annotation projects for a lunchtime talk series at Adams State College.
Download links:
YouTube Video- Last update: 11/04/2010
- Tags: Annotation Gene finding
